Related Experiment Video
Updated: Aug 12, 2025
Experimental Model of Ligature-Induced Peri-Implantitis in Mice
Published on: May 17, 2024
[Comparison of three methods for establishing rat peri-implantitis model]
1Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digi-tal Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Research Center of Engineering and Technology for Computerized Dentistry & NMPA Key Laboratory for Dental Materials, Beijing 100081, China.
Cotton thread ligation and Porphyromonas gingivalis lipopolysaccharide (LPS) injection, alone or combined, effectively induce peri-implantitis in rats. The combined method and cotton thread ligation showed faster and more significant marginal bone loss compared to LPS injection alone.
Area of Science:
- Periodontology
- Biomaterials Science
- Immunology
Background:
- Peri-implantitis, a significant complication of dental implants, requires reliable animal models for research.
- Current models for inducing peri-implantitis in rats have varying efficiencies.
- Understanding the inflammatory response, including cytokine expression, is crucial for peri-implantitis research.
Purpose of the Study:
- To compare the efficacy of cotton thread ligation, Porphyromonas gingivalis lipopolysaccharide (LPS) injection, and a combined approach in establishing a rat peri-implantitis model.
- To evaluate the speed and severity of peri-implantitis induction using these different methods.
- To assess marginal bone loss and inflammatory markers (TNF-α, IL-1β) in response to the induction methods.
Main Methods:
- Titanium implants were placed in rat maxillary molars, followed by a 4-week osseointegration period.
- Rats were divided into three groups: cotton thread ligation, LPS injection, and combined ligation/LPS.
- Peri-implantitis was induced, and samples were collected at 2 and 4 weeks for micro-CT analysis of bone loss and RT-qPCR for inflammatory cytokine expression. Clinical parameters (PD, BOP, GI) were recorded.
Main Results:
- All induction methods resulted in clinical signs of inflammation and marginal bone loss.
- Both cotton thread ligation and the combined method induced significantly greater marginal bone loss than LPS injection alone by 4 weeks.
- Expression of TNF-α and IL-1β was significantly higher in all test groups compared to controls at both time points.
Conclusions:
- Cotton thread ligation and the combined ligation/LPS methods are more efficient and faster for inducing peri-implantitis in rats compared to LPS injection alone.
- The combined approach may offer a more pronounced inflammatory response and bone loss, warranting further investigation.
- These findings provide a basis for selecting appropriate models for future peri-implantitis research.
