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Updated: Aug 11, 2025

Functional Calcium Imaging in Developing Cortical Networks
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[Measuring Neural Activity Using Calcium Indicators].

Masayuki Sakamoto1

  • 1Department of Optical Neural and Molecular Physiology, Graduate School of Biostudies, Kyoto University.

Brain and Nerve = Shinkei Kenkyu No Shinpo
|February 7, 2023
PubMed
Summary
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Genetically encoded calcium indicators enable precise measurement of neuronal activity. These advanced fluorescent probes, when used with multiphoton microscopy and microendoscopy, allow real-time, single-cell resolution monitoring in vivo.

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Imaging

Background:

  • Neuronal activity measurement is crucial for understanding brain function.
  • Traditional methods for monitoring neural activity have limitations in resolution and throughput.
  • Genetically encoded calcium indicators (GECIs) offer a powerful alternative for optical neuroimaging.

Approach:

  • This review details the features of advanced fluorescent calcium probes.
  • It explores the application of these probes in conjunction with multiphoton microscopy.
  • The use of microendoscopes for in vivo, single-neuron resolution imaging is discussed.

Key Points:

  • Development of highly sensitive and fast GECIs has advanced neuroscience.
  • Simultaneous measurement of neural activity from multiple neurons is now feasible.

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Last Updated: Aug 11, 2025

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  • Single-cell resolution in vivo imaging is achievable with current technologies.

    • Conclusions:

    • Fluorescent calcium probes are essential tools for modern neuroscience research.
    • The integration of GECIs with advanced microscopy techniques revolutionizes in vivo neural activity monitoring.
    • This approach provides unprecedented insights into neural circuits and dynamics.