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Related Experiment Video

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Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
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Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device.

Inmaculada Ortiz-Gómez1,2, Almudena Rivadeneyra2,3, José F Salmerón2,3

  • 1ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain.

Biosensors
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

A new microfluidic paper-based analytical device (µPAD) uses a near-field communication (NFC) tag for fast glutathione (GSH) determination. This portable, low-cost method offers high sensitivity for GSH detection in just 20 minutes.

Keywords:
NFC tagcolorimetric assayglutathione determinationhealth prognosissmartphoneµPAD

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Glutathione (GSH) is a crucial endogenous antioxidant involved in cellular defense mechanisms.
  • Accurate and rapid determination of GSH levels is vital for diagnosing and monitoring various diseases.
  • Existing GSH detection methods can be complex, time-consuming, or require specialized equipment.

Purpose of the Study:

  • To develop a portable, simple, and fast colorimetric method for glutathione (GSH) determination.
  • To implement a microfluidic paper-based analytical device (µPAD) integrated with a near-field communication (NFC) tag for GSH analysis.
  • To utilize smartphone technology for energy harvesting and image capture for quantitative GSH measurement.

Main Methods:

  • A colorimetric assay based on the redox reaction between Ag+, 3,3',5,5'-tetramethylbenzidine (TMB), and GSH.
  • Fabrication of a microfluidic paper-based analytical device (µPAD) incorporating an NFC tag.
  • Smartphone-powered energy harvesting to activate an LED for image capture of the µPAD for colorimetric analysis.

Main Results:

  • The developed method demonstrated a low detection limit of 1.0 µM for GSH.
  • The colorimetric signal (blue color fading) correlated with GSH concentration.
  • The entire determination process, from sample to result, was completed within 20 minutes.

Conclusions:

  • The proposed non-enzymatic method provides a highly sensitive, simple, fast, portable, and low-cost approach for GSH determination.
  • The integration of µPAD, NFC technology, and smartphones offers a promising platform for point-of-care diagnostics.
  • This innovative method facilitates efficient monitoring of GSH levels in various biological and environmental samples.