Related Experiment Video
Updated: Aug 9, 2025
10:17
Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
Published on: May 16, 2022
2.3K
3D-Structured and Blood-Contact-Safe Graphene Materials.
Beata Kaczmarek-Szczepańska1, Marta Michalska-Sionkowska2, Pawel Binkowski3
1Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
International Journal of Molecular Sciences
|February 25, 2023
Summary
This study presents an affordable 3D graphene preparation method using wet chemical exfoliation. The resulting graphene exhibits improved antioxidant properties and is nonhemolytic, making it suitable for biomedical applications like drug delivery.
Area of Science:
- Materials Science
- Biomedical Engineering
- Nanotechnology
Background:
- Graphene shows potential for biomedical applications, particularly in drug delivery.
- Developing cost-effective and scalable preparation methods for graphene is crucial for its widespread adoption.
- Assessing the biocompatibility of graphene materials, including antioxidant activity and hemocompatibility, is essential for safe biomedical use.
Purpose of the Study:
- To develop an inexpensive method for preparing 3D graphene via wet chemical exfoliation.
- To characterize the physicochemical properties of the prepared 3D graphene.
- To evaluate the antioxidant activity and hemocompatibility of the 3D graphene for potential biomedical applications.
Main Methods:
- 3D graphene was synthesized using wet chemical exfoliation.
- Morphological analysis was performed using Scanning Electron Microscopy (SEM) and High-Resolution Transmission Electron Microscopy (HRTEM).
- Elemental composition (C, N, H), Raman spectra, X-ray photoelectron spectroscopy (XPS), surface area, and pore volume were analyzed. Antioxidant activity was assessed using the DPPH method, and hemolysis rate was determined in contact with blood.
Main Results:
- The wet chemical exfoliation method yielded 3D graphene with characterized morphology and elemental composition.
- Thermal modification enhanced the radical scavenging activity (RSA), indicating improved antioxidant properties.
- All tested 3D graphene samples exhibited low hemolysis rates (0.28-0.64%), classifying them as nonhemolytic.
Conclusions:
- The developed wet chemical exfoliation method provides an inexpensive route to produce 3D graphene.
- The prepared 3D graphene demonstrates promising antioxidant and nonhemolytic properties.
- These findings support the potential of this 3D graphene material for biomedical applications, especially in drug delivery systems.
