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Updated: Aug 7, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Lanthanide-based luminescent probes for biological magnesium: accessing polyphosphate-bound Mg2
Brismar Pinto-Pacheco1, Qitian Lin1, Claudia W Yan1
1Department of Chemistry, New York University, 100 Washington Square East, New York, NY, 10003, USA. dbuccella@nyu.edu.
Abstract:
Biomolecule-bound Mg2+ species, particularly polyphosphate complexes, represent a large and dynamic fraction of the total cellular magnesium that is essential for cellular function but remains invisible to most indicators. Here we report a new family of Eu(III)-based indicators, the MagQEu family, functionalized with a 4-oxo-4H-quinolizine-3-carboxylic acid metal recognition group/sensitization antenna for turn-on, luminescence-based detection of biologically relevant Mg2+ species.
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