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An alternative fluorescence enhancement solution for use in lanthanide-based time-resolved fluoroimmunoassays
J A Keelan1, J T France, P M Barling
1Department of Obstetrics and Gynaecology, National Women's Hospital, Auckland, New Zealand.
Clinical Chemistry
|December 1, 1987
Summary
A new enhancement solution using thenoyltrifluoroacetone offers a cost-effective alternative for time-resolved fluoroimmunoassays. This commercially available beta-diketone enables reliable assay performance, similar to existing methods.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Time-resolved fluoroimmunoassays utilize lanthanide chelates as labels.
- These assays require an enhancement solution to form fluorescent lanthanide complexes.
- Existing solutions rely on 2-naphthoyltrifluoroacetone (NTA), which is not commercially available.
Purpose of the Study:
- To evaluate the performance of a novel enhancement solution based on commercially available thenoyltrifluoroacetone (TTA).
- To determine if TTA-based solutions are a viable alternative to NTA-based solutions in time-resolved fluoroimmunoassays.
Main Methods:
- Preparation of an enhancement solution using thenoyltrifluoroacetone (TTA).
- Application of the TTA-based solution in a commercial time-resolved fluoroimmunoassay.
- Comparison of results with those obtained using a standard NTA-based solution.
Main Results:
- The TTA-based enhancement solution yielded results comparable to the NTA-based solution.
- Fluorescence emission was approximately 27% lower with the TTA-based solution.
- Assay sensitivity was not significantly affected by the reduced fluorescence.
Conclusions:
- The TTA-based enhancement solution is a viable and economical alternative for time-resolved fluoroimmunoassays.
- This offers a practical option for laboratories developing their own lanthanide fluorescence-based assays.
- The commercial availability of TTA makes it particularly advantageous.