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Related Concept Videos

Subviral Agents01:29

Subviral Agents

59
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Viruses with RNA Genomes01:29

Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Retroviruses

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Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
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Related Experiment Video

Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
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Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

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Application of Pseudotyped Viruses.

Qianqian Cui1, Weijin Huang2

  • 1Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

Advances in Experimental Medicine and Biology
|March 15, 2023
PubMed
Summary
This summary is machine-generated.

Pseudotyped viruses offer a safer alternative for studying highly pathogenic viruses, enabling research in standard labs. This technology facilitates vaccine and antiviral drug development, advancing public health strategies against emerging infectious diseases.

Keywords:
ApplicationIn vitroIn vivoInfection mechanismNeutralizing antibodiesPseudotyped virus

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Area of Science:

  • Virology
  • Infectious Diseases
  • Biotechnology

Background:

  • Highly pathogenic viruses pose significant public health risks.
  • Traditional research requires high-level biosafety, limiting accessibility.
  • Pseudotyped viruses offer a safer, more accessible alternative for studying viral pathogens.

Purpose of the Study:

  • To provide an overview of pseudotyped virus technologies.
  • To highlight their applications in studying viral infection mechanisms and evaluating countermeasures.
  • To demonstrate their utility in research settings with limited biosafety infrastructure.

Main Methods:

  • Utilizing single-round infectious pseudotyped viruses that mimic native viral structures.
  • Employing pseudovirion-based neutralization assays for efficacy testing.
  • Establishing in vivo animal models for pseudotyped virus infections.

Main Results:

  • Pseudotyped viruses enable research on viral infection mechanisms in BSL-2 facilities.
  • They are effective tools for evaluating vaccines, monoclonal antibodies, and antiviral agents.
  • Applications include studying viral evolution, infectivity, antigenicity, and predicting immune responses.

Conclusions:

  • Pseudotyped virus technology is a versatile and valuable tool for infectious disease research.
  • It significantly broadens the scope of studies on highly pathogenic viruses.
  • Facilitates accelerated development of diagnostics, vaccines, and therapeutics.