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Related Concept Videos

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Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Related Experiment Video

Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
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Pseudotyped Viruses for Enterovirus.

Xing Wu1,2, Lisha Cui3, Yu Bai1,2

  • 1Division of Hepatitis Virus & Enterovirus Vaccines, Institute for Biological Products, National Institutes for Food and Drug Control, Beijing, China.

Advances in Experimental Medicine and Biology
|March 15, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a pseudotyped enterovirus (EVpv) using a non-pathogenic virus surrogate. This advancement enhances biosafety and aids in virological research, antibody detection, and disease monitoring.

Keywords:
EnterovirusNeutralizing antibodyPseudotyped virus

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Area of Science:

  • Virology
  • Biosafety
  • Molecular Biology

Background:

  • Non-pathogenic virus surrogates are crucial for safe scientific research, aligning with biosafety regulations.
  • Enteroviruses (EVs) are nonenveloped viruses, posing unique challenges for creating pseudotyped viral systems compared to enveloped viruses.
  • Pseudotyped viruses offer a safer alternative for studying wild-type viruses.

Purpose of the Study:

  • To detail the procedures and challenges in constructing pseudotyped enteroviruses (EVpv).
  • To highlight the utility of EVpv in fundamental virological studies.
  • To introduce applications of EVpv in serological monitoring and neutralizing antibody (NtAb) detection.

Main Methods:

  • Utilizing a non-pathogenic virus as a surrogate for enterovirus.
  • Developing customized construction methods for nonenveloped pseudotyped viruses.
  • Characterizing the resulting pseudotyped enterovirus (EVpv).

Main Results:

  • Successful construction of pseudotyped enterovirus (EVpv) was achieved.
  • The study outlines specific procedures and identifies challenges in EVpv preparation.
  • Demonstrated the applicability of EVpv in various research contexts.

Conclusions:

  • Pseudotyped enterovirus (EVpv) provides a viable and safer alternative for enterovirus research.
  • EVpv facilitates advancements in biosafety, virological studies, and diagnostic applications.
  • The described methods and applications pave the way for broader use of EVpv.