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Related Experiment Video

Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Replicating-Competent VSV-Vectored Pseudotyped Viruses.

Fei Yuan¹, Aihua Zheng²

¹State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Advances in Experimental Medicine and Biology

|March 15, 2023

Summary

This summary is machine-generated.

Vesicular stomatitis virus (VSV) can be engineered into a versatile live viral vector. By swapping its glycoprotein gene, researchers create recombinant VSV with altered cell tropism for vaccine and therapeutic applications.

Keywords:

Escape mutation screening Receptor screening Replicating-competent Vaccine development

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Area of Science:

Virology
Molecular Biology
Immunology

Background:

Vesicular stomatitis virus (VSV) is a prototype rhabdovirus.
Reverse genetics enables VSV genetic manipulation for use as a viral vector.

Purpose of the Study:

To describe the construction and applications of replicating-competent VSV (rcVSV) as a live viral vector.
To highlight how modifying the VSV envelope gene alters viral properties and applications.

Main Methods:

Genetic engineering of VSV by replacing the native glycoprotein gene with heterologous envelope genes.
Construction of recombinant VSV capable of replication in permissive cells.

Main Results:

Recombinant VSV particles incorporate foreign envelope proteins on their surface while maintaining VSV morphology.
The cell tropism of these recombinant VSV is dictated by the incorporated foreign envelope proteins.
Recombinant VSV successfully used in identifying viral receptors, screening antibody escape mutants, and developing vaccines.

Conclusions:

Replicating-competent VSV serves as a powerful and adaptable live viral vector platform.
VSV envelope modification allows for targeted cell tropism and diverse applications in virology and vaccinology.