A novel near-infrared fluorescent probe with large Stokes shift for imagining hydrogen sulfide

Hydrogen sulfide (H₂S) plays an important role in regulating varieties of important physiological and pathological processes. Thus the development of fluorescent probe for the detection of H₂S is of great significance and has attracted much attention recently. Herein, we reported a novel near-infrared (NIR) emitting fluorescent probe WFP-PC, which contained a positive charged hemicyanine-based WFP-OH as fluorophore and thiobenzoate unit as a specific reaction site. After treated with H₂S, the probe exhibited significant fluorescence enhancement and response time within 4 min and detection limit as low as 0.47 μM, accompanied by color changes from purple to blue. The probe was successfully applied to imaging the exogenous/endogenous H₂S in cells and mice, suggesting it could be a promising molecular tool for H₂S detection in living systems.