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Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization.

Nina Lokar1, Borut Pečar1, Matej Možek1

  • 1Laboratory of Microsensor Structures and Electronics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, Slovenia.

Biosensors
|March 29, 2023
PubMed
Summary

This study presents a microfluidic electrochemical glucose biosensor using PQQ-GdhB enzyme immobilization. The developed biosensor offers a sensitive and reliable method for continuous glucose monitoring.

Keywords:
chronoamperometrycyclic voltammetryelectrochemical biosensorenzyme in situ immobilizationmicrofluidic glucose biosensorthin film electrode

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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Microfluidics

Background:

  • Development of sensitive and reliable glucose biosensors is crucial for diabetes management.
  • Microfluidic platforms offer advantages for integrated and miniaturized analytical devices.

Purpose of the Study:

  • To develop and characterize a novel microfluidic electrochemical glucose biosensor.
  • To investigate the in situ enzyme immobilization technique for enhanced biosensor performance.

Main Methods:

  • Fabrication of thin-film metal electrodes on a glass substrate.
  • In situ immobilization of pyrroloquinoline quinone-glucose dehydrogenase (PQQ-GdhB) enzyme using microcontact printing.
  • Integration of microfluidic channels in polydimethylsiloxane (PDMS).
  • Electrochemical characterization using cyclic voltammetry and chronoamperometry.

Main Results:

  • The biosensor demonstrated a linear range up to 200 μM and a measurement range up to 10 mM glucose.
  • A low detection limit of 30 µM glucose was achieved.
  • Sensitivities of 0.79 nA/µM/mm² (with flow) and 0.61 nA/µM/mm² (without flow) were obtained at 20 µL/min.

Conclusions:

  • The developed microfluidic glucose biosensor with in situ enzyme immobilization shows promising performance.
  • This approach facilitates continuous and time-independent glucose detection.
  • The methodology is applicable to a wide range of microfluidic biosensor development.