Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

54.9K
Overview
54.9K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

53
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
53
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

51
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
51
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

100
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
100
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pyocyanin produced by <i>Pseudomonas aeruginosa</i> creates legacy effects that promote antibiotic resistance evolution in enterococci.

Antimicrobial agents and chemotherapy·2026
Same author

Emergent Homeostasis and Degeneracy From Multi-Dimensional Attractors.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same author

Jumbo phage-mediated transduction of genomic islands.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Living Together Apart: Quantitative Perspectives on the Costs and Benefits of a Multipartite Genome Organization in Viruses.

Viruses·2025
Same author

Density-dependent feedback limits the spread of beta-lactamase mutants: experimental observations and population dynamic model.

mBio·2025
Same author

An extreme mutational hotspot in nlpD depends on transcriptional induction of rpoS.

PLoS genetics·2025
Same journal

Bridging two hosts: how intracellular environments shape flaviviral infection.

mBio·2026
Same journal

Post-translational negative feedback loops are sufficient to coordinate synthesis of the gram-negative envelope during steady-state growth.

mBio·2026
Same journal

mGem: A tale as old as blood-do tick-borne pathogens exploit arthropod antioxidant defenses?

mBio·2026
Same journal

mGem: Subcellular compartments in bacterial pathogens and their role during infection.

mBio·2026
Same journal

mGem: A perfect storm in the era of global warming-the convergence between thermotolerant fungi and altered immunity.

mBio·2026
Same journal

Global genomic surveillance of <i>Salmonella</i> in the environment: assessing virulence and antimicrobial resistance at scale.

mBio·2026
See all related articles

Related Experiment Video

Updated: Aug 4, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.8K

Minimal Surviving Inoculum in Collective Antibiotic Resistance.

Lukas Geyrhofer1, Philip Ruelens2,3, Andrew D Farr2,4

  • 1Technion-Israel Institute of Technology, Haifa, Israel.

Mbio
|April 6, 2023
PubMed
Summary
This summary is machine-generated.

Bacteria collectively resist antibiotics by degrading them, a process crucial for population survival. Survival depends on the balance between bacterial death rates and antibiotic removal rates, not specific molecular details.

Keywords:
antibiotic resistancebacterial collective dynamicsbeta-lactamaseinoculum effectmathematical modelingpopulation dynamics

More Related Videos

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

2.0K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K

Related Experiment Videos

Last Updated: Aug 4, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.8K
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

2.0K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K

Area of Science:

  • Microbiology
  • Theoretical Biology
  • Mathematical Modeling

Background:

  • Antibiotic resistance is a significant clinical challenge.
  • Bacterial enzymatic degradation of antibiotics is a key resistance strategy.
  • Collective resistance mechanisms at the population level require further quantitative understanding.

Purpose of the Study:

  • To develop a theoretical framework for collective antibiotic resistance via degradation.
  • To identify key parameters governing population survival.
  • To propose and validate an experimental assay for measuring collective resistance.

Main Methods:

  • Development of a general theoretical framework using mathematical modeling.
  • Coarse-grained phenomenological modeling focusing on timescales and cooperativity.
  • Experimental validation using Escherichia coli and β-lactamase expression, measuring minimal surviving inoculum.

Main Results:

  • Population survival is critically dependent on the ratio of population death and antibiotic removal timescales.
  • Resistance is largely insensitive to specific molecular, biological, or kinetic details.
  • A simple experimental assay effectively measures dose-dependent minimal surviving inoculum.

Conclusions:

  • Collective antibiotic degradation is a robust survival strategy influenced by broad kinetic parameters.
  • The developed framework and assay provide a mechanism-independent approach to studying antibiotic resistance.
  • Findings can inform strategies for combating antibiotic resistance in complex microbial communities.