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Boronic Acid Resin for Selective Immobilization of Canonically Encoded Proteins
James O Larkin1, Brianna Jayanthi2, Laura Segatori2
1Department of Chemistry, Rice University, 6100 Main Street, Houston, Texas 77005, United States.
Abstract:
The use of transition-metal-mediated boronic acid chemistry presents a novel method of protein immobilization on a solid support. This is a one-step method that site-selectively immobilizes pyroglutamate-histidine (pGH)-tagged proteins. Herein, we describe the synthesis of alkenylboronic acid-functionalized poly(ethylene glycol) acrylamide (PEGA) resin and its subsequent reactions with pGH-tagged proteins to produce covalent linkages. The selectivity of immobilization is demonstrated within fluorescent studies, model mixtures, and lysates.
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