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Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research
Manjusha Vaidya1, Nasif Sayeed1, Caroline Hobson1
1Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2023
Summary
Extracellular vesicles (EVs) are natural carriers of molecules that can alter cells. Researchers are customizing these EVs for targeted delivery, especially across the blood-brain barrier for brain research.
Area of Science:
- Biotechnology
- Neuroscience
- Cell Biology
Background:
- Extracellular vesicles (EVs) mediate intercellular communication by transferring nucleic acids, proteins, and lipids.
- EV cargo can induce genetic, physiological, and pathological changes in recipient cells.
- EVs possess the inherent capacity to cross the blood-brain barrier (BBB).
Purpose of the Study:
- To explore the customization of EVs for targeted cargo delivery.
- To highlight the potential of EVs as drug delivery vehicles for brain research.
- To provide laboratory techniques for EV customization in neuronal studies.
Main Methods:
- Laboratory protocols for EV isolation and characterization.
- Techniques for modifying EV surface or cargo.
- Methods for assessing EV targeting and uptake in neuronal models.
Main Results:
- Demonstrated EV customization for specific organ and cell-type targeting.
- Validated the use of EVs for delivering therapeutic payloads across the BBB.
- Established protocols for neuronal research applications.
Conclusions:
- EVs are versatile natural nanocarriers with significant therapeutic potential.
- Customization strategies enable precise control over EV cargo and targeting.
- EV-based delivery systems offer a promising approach for treating neurological disorders.
