Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.8K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable, High-Relaxivity Gd(III)-Conjugated Lipoic Acid Hydrogels for Magnetic Resonance Imaging.

ACS applied materials & interfaces·2026
Same author

An Enzyme-Responsive Gd(III) MRI Probe for Visualizing β-Hexosaminidase A Activity.

ACS sensors·2026
Same author

Nickel binding shifts Helicobacter pylori HypA toward compact conformations.

Journal of inorganic biochemistry·2026
Same author

Moving NMR infrastructures to remote access capabilities.

Progress in nuclear magnetic resonance spectroscopy·2026
Same author

Recent Trends in Metabolomics by NMR Spectroscopy.

Angewandte Chemie (International ed. in English)·2026
Same author

Machine learning supported olive oil compound profiling for assessing geographic and cultivar authenticity.

Food research international (Ottawa, Ont.)·2026

Related Experiment Video

Updated: Jul 31, 2025

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

14.9K

Practical considerations for rapid and quantitative NMR-based metabolomics.

Frans A A Mulder1, Leonardo Tenori2, Cristina Licari2

  • 1Johannes Kepler Universität, Linz, Austria.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|May 4, 2023
PubMed
Summary
This summary is machine-generated.

Nuclear Magnetic Resonance (NMR) spectroscopy can be faster for metabolomics. Adding gadolinium chelates and optimizing temperature control reduces experiment times, making NMR metabolomics more efficient.

Keywords:
MetabolomicsNMR data acquisitionTemperature stabilizationbucketingparamagnetic NMR

More Related Videos

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

597
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

Related Experiment Videos

Last Updated: Jul 31, 2025

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

14.9K
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

597
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is crucial for metabolomics due to its reliability and reproducibility.
  • Long spin relaxation times (T1) of small molecules in NMR limit high-throughput data acquisition, increasing experimental time.
  • Inefficient temperature regulation during sample exchange further hinders rapid analysis in NMR metabolomics.

Purpose of the Study:

  • To present practical strategies for enhancing the efficiency and utility of NMR spectroscopy in metabolomics.
  • To address the limitations of long relaxation times and slow temperature regulation in high-throughput NMR analysis.
  • To introduce methods for faster data acquisition and processing in NMR-based metabolomic studies.

Main Methods:

  • Addition of paramagnetic gadolinium chelate to accelerate spin relaxation times for small molecules.
  • Optimization of temperature regulation protocols to minimize idle time during sample exchanges.
  • Implementation of equidistant bucketing for rapid metabolomic fingerprinting.

Main Results:

  • Gadolinium chelate addition enables cost-effective, high-throughput mixture analysis with accurate concentration determination.
  • Optimized temperature control reduces NMR sample scanning times by an additional factor of two.
  • Equidistant bucketing provides a simple and fast method for metabolomic fingerprinting.

Conclusions:

  • The integration of gadolinium-assisted relaxation, optimized temperature control, and equidistant bucketing significantly enhances NMR metabolomics efficiency.
  • These advancements make NMR spectroscopy a more versatile and high-throughput tool for metabolomic studies.
  • The proposed methods address key bottlenecks, paving the way for broader adoption of NMR in metabolomics research.