MICROSTRUCTURE OF BIOPOLYMER MICRO-FIBROUS SCAFFOLD AND ITS INFLUENCE ON THE ABILITY TO RETAIN MEDICINES AND TISSUE...

The aim of the research is to study the microstructure, antibiotic-absorbing and framework capacity of the fibrous non-woven PCL matrices designed by us for the regeneration of tissues and capillaries. Samples of microfibrous non-woven matrices made by our technology out of polycaprolactone PCL (invention patent of Ukraine № 119958) were used in the work. Antibiotic retention in samples of matrix materials was evaluated during the 1st, 3rd, 5th, 7th, 14th, 18th and 21st days of the experiment. The experimental part of the research was performed using 30 laboratory animals (rabbits). On the basis of microscopic studies of the biopolymer microfiber matrices obtained by us, the relationship between the increase in polymer concentration in the sucrose melt and the increase in the percentage of thicker microfibers was determined. Microbiological analysis of the antibiotic-absorbing capacity of the obtained microfibrous biopolymer non-woven matrices determined that lincomycin impregnated into polymer matrices is characterized by less stability during storage than cefazolin. Antibiotic concentrations of the impregnated matrix material samples were actively maintained at the level of control values for a period of 5 days. The pathomorphological analysis of soft tissues at all times of subcutaneous implantation in the experiment made it possible to determine the fact of regeneration of tissues and the microcirculatory channel through the entire thickness of the fibrous matrix. This was confirmed by a significant decrease in the area of the connective tissue matrix per vessel from (49345.18+485.63) μm2 to (24797.47+480.28) μm2, an increase in the cross-sectional area of vessels from (697.61+21.79) μm2 to (1321.23+24.82) μm2 and a decrease in the thickness of vascular walls from (3.2+0.05) μm to (2.65+0.07) μm (p<0.01) from the periphery to the center of the frame. These facts, in our opinion, confirm the framework function of the polymer matrix synthesized by us, which is also a means of one-time local delivery of the medicine to the tissues in the damaged area.