Long-term imaging of intranuclear Mg²⁺ dynamics during mitosis using a localized fluorescent probe

A novel fluorescent Mg²⁺ probe was developed based on a small molecule-protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg²⁺ over Ca²⁺. Using ratiometric fluorescence microscopy with a co-localized standard fluorophore, the variations in intranuclear Mg²⁺ concentrations during mitosis could be visualized.