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Updated: Jul 29, 2025
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Physicochemical Properties and Biocompatibility of Various Bioceramic Root Canal Sealers: In Vitro Study
Sang Won Kwak1, Jina Koo2, Minju Song3
1Department of Conservative Dentistry, Dental Research Institute, Pusan National University, School of Dentistry, Yangsan, Korea; Dental and Life Science Institute, Pusan National University, School of Dentistry, Yangsan, Korea.
Introduction:
The aim of this study was to compare the physicochemical properties and biocompatibility of various calcium silicate-based bioceramic sealers (CSBSs).
Methods:
Four recently developed CSBSs, including AH Plus Bioceramic Sealer (AHB), EndoSequence BC Sealer (ESB), TotalFill BC Sealer (TTB), and Bio-C Sealer (BIC), were compared with the epoxy resin-based sealer AH Plus (AHP). Their physical properties, including flow, setting time, radiopacity, dimensional stability, and pH, were evaluated according to the International Organization for Standardization (ISO) 6876. Their cytotoxicity in human periodontal ligament fibroblast (hPDLF) was assessed through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and compared. Additionally, cell attachment to the sealer surface was analyzed using green fluorescent protein and confocal laser scanning microscopy to evaluate cell viability. Data were analyzed using a one-way analysis of variance to determine the difference between groups for categorical variables, followed by Tukey's post hoc test at the significance level of 95%.
Results:
The flow, setting time, and radiopacity of all tested CSBSs satisfied the ISO 6876/2012 standards. Further, these CSBSs showed shrinkage after immersion in distilled water for 30 days and complied with the ISO 6876/2001 requirements. The pH values of AHB, ESB, TTB, and BIC were greater than 11, whereas AHP had a pH of 6.69 after 4 weeks. CSBS showed excellent biocompatibility compared with that of AHP (P < .05). Confocal laser scanning microscopy showed that alive hPDLFs were attached well to all the tested CSBSs but not to AHP.
Conclusions:
CSBSs have similar physical characteristics within the ISO standards and higher biocompatibility than epoxy resin-based sealers.
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