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IN VITRO EFFECT OF SUCROSE FEEDING IN THE DIFFERENTIATION OF WOOD ELEMENTS OF PLUMERIA
Lily Ray1, Srilekha Ghosh1, P C Datta1
1Botanical Lab. of Pharmaco-anatomy, University of Calcutta, Calcutta-700019, India.
Development, Growth & Differentiation
|June 7, 2023
Summary
Plumeria rubra Linn. wood cambium cultured in vitro with sucrose showed similar differentiation to in situ growth. Sucrose promoted cell enlargement and increased lignified elements, enhancing wood development.
Area of Science:
- Plant biology
- Wood science
- Plant tissue culture
Background:
- Understanding wood formation (secondary growth) is crucial for forestry and material science.
- Plumeria rubra Linn. (temple tree) is a tropical plant with potential for ornamental and medicinal uses.
Purpose of the Study:
- To investigate the effect of in vitro culture conditions on wood cambium differentiation in Plumeria rubra Linn.
- To compare in vitro wood development with in situ growth.
Main Methods:
- Wood cambium explants of Plumeria rubra Linn. were cultured for 75 days in a modified Schenk and Hildebrandt medium.
- In vitro cell dimensions and frequencies were compared to those of in situ grown tissues.
Main Results:
- In vitro culture with 41% sucrose induced differentiation comparable to in situ growth.
- A significant increase in non-reducing sugar content was observed in tissues grown in situ (0.0004% to 0.0032%).
- Sucrose promoted the enlargement of axial elements and increased the number of lignified elements in vitro.
Conclusions:
- Sucrose is a key factor influencing wood cambium differentiation in Plumeria rubra Linn. under in vitro conditions.
- In vitro culture can effectively mimic natural wood formation processes, offering a model for studying secondary growth.
