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The RNA-Binding Proteins OAS1, ZFP36L2, and DHX58 Are Involved in the Regulation of CD44 mRNA Splicing in Colorectal

V O Novosad1,2, D V Maltseva3,4

  • 1Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia.

Bulletin of Experimental Biology and Medicine
|June 19, 2023
PubMed

Insights

This study identifies novel RNA-binding proteins regulating CD44 protein isoforms crucial for cancer. OAS1, ZFP36L2, and DHX58 are newly found regulators of CD44 isoforms 3 and 4.

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Cancer Research

Background:

  • Alternative splicing is regulated by RNA-binding proteins, influencing cellular functions.
  • Transmembrane protein CD44 is vital in cancer metastasis and a target for therapies and prognostic markers.
  • Specific CD44 isoforms have distinct functions, necessitating targeted study.

Purpose of the Study:

  • To identify RNA-binding proteins that regulate clinically significant CD44 protein isoforms 3 and 4.
  • To explore novel regulators of CD44 alternative splicing.

Main Methods:

  • Bioinformatic analysis was employed to search for RNA-binding proteins.
  • The study focused on identifying regulators of CD44 isoforms 3 and 4.

Main Results:

  • Five RNA-binding proteins were identified as potential regulators of CD44 isoforms 3 and 4.
  • OAS1, ZFP36L2, and DHX58 were identified as novel regulators in this context.

Conclusions:

  • The findings reveal new regulatory mechanisms for CD44 alternative splicing.
  • OAS1, ZFP36L2, and DHX58 represent potential therapeutic targets or biomarkers in cancer.
Keywords:
CD44 alternative splicingDHX58OAS1RNA-binding proteinsZFP36L2

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