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Platelet function analyzer-200 closure curve analysis and assessment of flow-obstructed samples
Matthew R Kornya1, Anthony C G Abrams-Ogg1, Shauna L Blois1
1Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Veterinary Clinical Pathology
|July 12, 2023
Summary
Numerical parameters accurately detect clopidogrel effects in feline blood samples with flow obstruction. Visual analysis of Platelet Function Analyzer-200 curves was unreliable for determining clopidogrel
Area of Science:
- Veterinary Hematology
- Pharmacodynamics
- Diagnostic Assay Development
Background:
- The Platelet Function Analyzer-200 (PFA-200) assesses clopidogrel efficacy in cats.
- Flow obstruction in PFA-200 assays yields uninterpretable results.
- Closure curves and parameters like initial flow rate (IF) and total volume (TV) may aid interpretation.
Approach:
- Reviewed PFA-200 closure curves to identify flow obstruction.
- Utilized non-obstructed curves to establish clopidogrel effect benchmarks.
- Evaluated IF, TV, and primary hemostasis components (PHC) for categorizing clopidogrel effects.
- Assessed blinded observers' ability to visually interpret curves for clopidogrel impact.
Key Points:
- Numerical parameters (TV, PHC(1), PHC(2)) accurately predicted closure in flow-obstructed samples (AUC 0.79-0.87).
- Visual curve analysis demonstrated low accuracy (55%) and poor inter-reviewer agreement (Fleiss' Kappa 0.06).
Conclusions:
- Visual assessment of PFA-200 curves is insufficient for determining clopidogrel's effect in obstructed feline samples.
- Quantitative analysis of PFA-200 parameters reliably detects clopidogrel's impact even with flow obstruction.
