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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System.

Megan M Foley1

  • 1Department of Forensic Sciences, The George Washington University, Washington, DC, USA. mmfoley@gwu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2023
PubMed
Summary
This summary is machine-generated.

The RapidHIT™ ID System generates a CODIS-compatible DNA profile in 90 minutes. This automated system simplifies forensic DNA analysis for law enforcement and disaster victim identification.

Keywords:
FBI DISCGeneMarker™ HIDGlobalFiler™ ExpressNGM SElect™ ExpressRapid DNARapid STR AnalysisRapidHIT™ IDRapidINTEL™RapidLINK™

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Traditional forensic DNA analysis can be time-consuming.
  • The need for rapid DNA profiling in various field applications is increasing.
  • Current systems may require specialized laboratory personnel.

Purpose of the Study:

  • To describe the RapidHIT™ ID System for rapid forensic DNA profile generation.
  • To detail the workflow from sample to profile using the system.
  • To introduce the associated software for analysis and management.

Main Methods:

  • Utilizes a direct amplification workflow targeting CODIS or ESS core loci.
  • Employs preloaded cartridges and a fully automated workflow.
  • Features a user-friendly interface for single sample processing.

Main Results:

  • Generates a CODIS-compatible Short Tandem Repeat (STR) profile in 90 minutes.
  • The RapidLINK™ Software provides initial analysis and flags non-compliant profiles.
  • Enables real-time results management for multi-instrument/multi-location systems.

Conclusions:

  • The RapidHIT™ ID System offers a rapid, automated solution for forensic DNA profiling.
  • The system is suitable for field use by non-laboratory personnel, such as law enforcement.
  • Facilitates timely database hits for investigations and aids in mass disaster victim identification.