Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordination-Driven Metal-Phenolic Network Complexes for Programmable Colorimetric Signal Transduction and Defined Nano-Bio Interfaces.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

ANXA11 recruits tumor-associated neutrophils to promote the progression of colorectal cancer.

International immunopharmacology·2026
Same author

Whole blood exchange-lymphoplasmapheresis combined transfusion as an immunotherapy in systemic lupus erythematosus.

iScience·2026
Same author

An LLM-agent-based framework for calculating nodal carbon intensity in regional power systems.

Scientific reports·2026
Same author

Preparation and eye-protecting application of lutein Pickering emulsions stabilized by tea polyphenol/cassia seeds.

Food chemistry·2026
Same author

Curcumin-loaded Pickering emulsion stabilized by Spanish mackerel meat-hyaluronic acid and its application in Spam.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: Jul 23, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K

Computer Vision-Based Artificial Intelligence-Mediated Encoding-Decoding for Multiplexed Microfluidic Digital

Weiqi Zhao1, Yang Zhou1,2, Yao-Ze Feng2

  • 1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei China.

ACS Nano
|July 17, 2023
PubMed
Summary

A novel multidimensional digital immunoassay uses microparticle encoding and AI decoding for sensitive, multiplexed detection. This technology offers rapid, accurate analysis of multiple targets, advancing biosensing applications.

Keywords:
biomarkercomputer visiondigital immunoassaymachine learningmicrofluidicsmultiplexed detection

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.6K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.2K

Related Experiment Videos

Last Updated: Jul 23, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.6K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.2K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Artificial Intelligence

Background:

  • Digital immunoassays are crucial for clinical diagnosis, food safety, and environmental monitoring, demanding multiplexed capacity, high sensitivity, and affordability.
  • Current methods often face limitations in multiplexing, sensitivity, or operational convenience.

Purpose of the Study:

  • To develop a multidimensional digital immunoassay integrating microparticle-based encoding and AI-based decoding.
  • To achieve sensitive, multiplexed detection of multiple targets with convenient operation.

Main Methods:

  • Utilized microparticle encoding based on size, number, and color for multiplexed information storage.
  • Employed computer vision-based artificial intelligence for image analysis, decoding encoded information, and visual output.
  • Integrated optical microscopy imaging with a microfluidic platform for seamless encoding-decoding.

Main Results:

  • Successfully developed a multidimensional digital immunoassay capable of simultaneous analysis of multiple targets.
  • Demonstrated high sensitivity and a broad detection range (pg/mL to μg/mL) for inflammatory markers and antibiotics.
  • Achieved analysis within 30 minutes, showcasing convenient operation.

Conclusions:

  • The developed microfluidic digital immunoassay offers a powerful platform for next-generation multiplexed biosensing.
  • The integration of AI-based decoding with microparticle encoding provides an intelligent and efficient bioassay solution.
  • This technology holds significant promise for various applications requiring rapid and sensitive multiplexed detection.