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Exploring Enzymatic Conformational Dynamics at Surfaces through μ-FTIR Spectromicroscopy.
Giovana Rossi Mendes1, Iago de Assis Modenez1, Giovana Rosso Cagnani1
1São Carlos Institute of Chemistry, University of São Paulo, São Carlos 13560-970, Brazil.
Analytical Chemistry
|July 17, 2023
Summary
Investigating enzyme immobilization, this study uses infrared spectromicroscopy to map protein conformational changes on various surfaces. This reveals how enzyme structure adapts to solid supports, crucial for biosensing and bioenergy applications.
Area of Science:
- Biophysical Chemistry
- Surface Science
- Biomaterials Engineering
Background:
- Protein immobilization on solid supports is vital for industrial, technological, and medical applications.
- Enzyme-surface interactions cause conformational changes affecting catalytic activity and stability.
- Existing methods like circular dichroism and nonlinear laser spectroscopy have limitations for surface-bound proteins.
Purpose of the Study:
- To develop and apply a reliable method for probing enzyme conformational changes on solid surfaces.
- To investigate the spatial dynamics of enzyme adsorption and structural adaptation.
- To understand how different surface properties influence enzyme behavior.
Main Methods:
- Utilized high-dimensional data spectromicroscopy analysis in the infrared region (μ-FTIR).
- Mapped enzyme conformational changes across a 20 mm² area on model substrates (CaF₂, Au, Au-thiol).
- Employed Alcohol dehydrogenase (ADH) as a model enzyme.
Main Results:
- Demonstrated the ability to map enzyme conformational dynamics on diverse solid surfaces.
- Observed different adsorption patterns and dynamic secondary structure adjustments.
- Showcased how enzymes adapt to optimize interprotein and enzyme-surface interactions.
Conclusions:
- μ-FTIR provides a viable approach for investigating immobilized protein structure and dynamics.
- Understanding enzyme-surface interactions is key for optimizing immobilized enzyme performance.
- Findings offer insights for advancing biosensing, bioenergy, and other biomaterial applications.
