Related Experiment Video
Updated: Jul 22, 2025
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
14.0K
Advances in Flow Cytometry for Mixed Phenotype and Ambiguous Leukemias
Jason H Kurzer1, Olga K Weinberg2
1Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, H1524B, Stanford, CA 94305-5324, USA.
Clinics in Laboratory Medicine
|July 22, 2023
Summary
This review covers new diagnostic approaches for acute leukemias of ambiguous lineage, highlighting flow cytometry and genetic findings. It details characteristic immunophenotypes for novel entities like mixed phenotype acute leukemia with ZNF384 rearrangements.
Area of Science:
- Hematology
- Oncology
- Immunophenotyping
Background:
- Acute leukemias of ambiguous lineage present diagnostic challenges.
- Accurate classification is crucial for appropriate treatment and prognosis.
- Flow cytometry is a key diagnostic tool, but interpretation requires careful consideration.
Purpose of the Study:
- To review recent updates in the diagnosis of acute leukemias of ambiguous lineage.
- To emphasize essential elements for proper flow cytometric evaluation.
- To discuss characteristic immunophenotypes of novel entities.
Main Methods:
- Review of current literature and classification systems.
- Analysis of flow cytometric marker expression patterns.
- Integration of immunophenotypic and genetic findings.
Main Results:
- Current classification emphasizes marker intensity and genetic data over sole immunophenotyping for mixed phenotype acute leukemia.
- Novel entities, including mixed phenotype acute leukemia with ZNF384 rearrangements and acute leukemias of ambiguous lineage with BCL11B rearrangements, exhibit distinct flow cytometric profiles.
- Characteristic immunophenotypes for these novel entities are detailed.
Conclusions:
- Proper diagnosis of acute leukemias of ambiguous lineage requires a comprehensive approach integrating flow cytometry and genetic information.
- Understanding specific immunophenotypes aids in identifying novel entities and refining classification.
- This review provides essential insights for accurate diagnosis and management.
