Related Experiment Video
Updated: Jul 21, 2025
11:26
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
13.7K
Engineering Biological Nanopore Approaches toward Protein Sequencing.
Xiaojun Wei1,2, Tadas Penkauskas3,4, Joseph E Reiner5
1Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States.
ACS Nano
|July 25, 2023
Summary
Nanopore technology shows promise for single-molecule protein sequencing, overcoming current limitations in protein identification. Further engineering and interdisciplinary collaboration are key to realizing its potential for analyzing individual amino acids and proteins.
Area of Science:
- Biotechnology
- Nanotechnology
- Biophysics
Background:
- Current protein studies are limited by inadequate single-molecule identification and quantification methods.
- Nanopore systems, successful in genome sequencing, are now being explored for protein analysis.
Purpose of the Study:
- To review recent advancements in biological nanopore sensors for protein sequencing.
- To provide engineering-focused recommendations for developing nanopore-based protein sequencing.
Main Methods:
- Review of recent advances in biological nanopore sensors.
- Analysis of device and algorithm development for protein translocation and identification.
- Utilizing molecular simulations to understand underlying mechanisms.
Main Results:
- Nanopore systems demonstrate potential for protein identification, detection, and analysis at the single-molecule level.
- Progress has been made in identifying individual amino acids and controlling peptide/protein translocation.
Conclusions:
- Nanopore-based protein sequencing offers realistic prospects for novel analytical capabilities.
- Advancement requires interdisciplinary collaboration, including chemical conjugation, protein engineering, simulation, machine learning, and device fabrication.
Related Concept Videos
03:21RNA-seq
10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
01:57Sanger Sequencing
754.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.8K
03:00Next-generation Sequencing
91.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.6K