Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.8K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.2K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

67
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
67
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

81
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
81

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ITM2B-associated retinal dystrophy mutation modifies BRI23 peptide interactions in the human retina.

Scientific reports·2026
Same author

<i>In vivo</i> characterization of a retinal cellular biomarker of inflammation in multiple sclerosis.

Brain communications·2026
Same author

Imaging of Tissue and Cell Dynamics: introduction to the feature issue.

Biomedical optics express·2026
Same author

Influence of retinal eccentricity, color blindness, and age on optoretinography measured with AOSLO.

Biomedical optics express·2025
Same author

Wide-field cellular-resolution retinal imaging using deformable mirror-based sensorless adaptive optics time-domain full-field OCT.

Biomedical optics express·2025
Same author

Rapid spectral shaping for time domain and swept source full field OCT.

Biomedical optics express·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Jul 21, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K

Dynamic optical coherence tomography for cell analysis [Invited].

Salvatore Azzollini1, Tual Monfort1,2, Olivier Thouvenin3

  • 1Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France.

Biomedical Optics Express
|July 27, 2023
PubMed
Summary
This summary is machine-generated.

Dynamic optical coherence tomography (D-OCT) enables label-free live imaging of cellular dynamics. This review compares D-OCT techniques, applications, and future potential for evaluating cell physiology in 3D tissue samples.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.7K

Related Experiment Videos

Last Updated: Jul 21, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.7K

Area of Science:

  • Biomedical Optics
  • Cellular Imaging
  • Optical Coherence Tomography

Background:

  • Label-free live optical imaging is crucial for studying dynamic cellular processes.
  • Advances in dynamic optical coherence tomography (D-OCT) have enabled new imaging capabilities.
  • D-OCT analyzes intracellular organelle movement to assess cell motility and metabolic state.

Purpose of the Study:

  • To review and compare various dynamic optical coherence tomography (D-OCT) techniques.
  • To provide an overview of current applications of D-OCT in biological research.
  • To discuss the future outlook and opportunities for D-OCT in live cell imaging.

Main Methods:

  • Comparison of different D-OCT methodologies.
  • Analysis of temporal fluctuations in optical signals.
  • Ensemble metric calculation for cell motility and metabolic state.

Main Results:

  • D-OCT allows visualization of cells in static environments and evaluation of their physiology.
  • Emerging D-OCT techniques show promise for 3D evaluation of live tissue samples, including biopsies and cell cultures.

Conclusions:

  • D-OCT is a powerful tool for label-free, live imaging of cellular dynamics.
  • The technique offers significant potential for 3D assessment of tissue physiology.
  • Continued development of D-OCT promises expanded applications in biomedical research.