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Updated: Jul 16, 2025

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
Published on: June 5, 2017
Techniques in Staining of Rodent and Human Olfactory Tissue
Hironobu Nishijima1, Eric H Holbrook2
1Department of Otolaryngology-Head and Neck Surgery, The University of Tokyo, Tokyo, Japan.
Abstract:
Staining of olfactory tissue allows for evaluation of the organization of specific cell types within the specimen and allows for assessment in abnormalities of function that may be related to changes in normal tissue architecture. Histochemical staining and immunohistochemical (IHC) techniques are the most common methods for visualizing olfactory epithelium and olfactory bulbs. Here we describe the method of IHC for olfactory tissue utilizing both fluorescent and peroxidase-based methods of visualization.
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