[Citrate metabolism during the consolidation of experimental fractures]

Simulation of reparative osteogenesis in diaphysis fracture of rat femur showed that liver tissue citrate synthase was a key enzyme in metabolism of citrate under conditions of organic matrix development in regenerating tissue. The liver enzyme as well as the callus citrate synthase were responsible for high rate of lipogenesis (activation of ATP-citrate lyase) and for an increase in content of citric acid within the initial period of consolidation. At the late steps of reparation callus citrate synthase, involved in citrate metabolism, promoted activation of energy-dependent reactions of calcification (stimulation of NAD-isocitrate dehydrogenase) and determined the dynamics of the metabolite concentration.