[A limiting factor of glycolysis in bone and callus]

Phosphofructokinase reaction was shown to be a key step of glycolysis after addition of various substrates to postmitochondrial fraction of bone and callus in vitro. Administration of fructose-1,6-diphosphate into the animals with femur fracture within a first week of the fracture consolidation led to activation of glycolysis in regenerate as a result of which more mature callus was formed within 10 weeks.