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Updated: Aug 12, 2026

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
Published on: March 14, 2014
Transcriptome-Wide Analysis of mRNA Adenylation Status in Yeast Using Nanopore Sequencing
Pawel S Krawczyk1, Agnieszka Tudek2, Seweryn Mroczek1,3
1International Institute of Molecular and Cell Biology, Warsaw, Poland.
Abstract:
There are multiple methods for studying deadenylation, either in vitro or in vivo, which allow for observation of mRNA abundance or poly(A) tail dynamics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast Saccharomyces cerevisiae using ONT.
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