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Updated: Jul 11, 2025

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
Published on: October 17, 2013
Albumin Retains Its Transport Function after Interaction with Cerium Dioxide Nanoparticles
Madina M Sozarukova1, Ekaterina M Kochneva2, Elena V Proskurnina3
1Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Avenue, 31, Moscow 119991, Russia.
Interactions between cerium dioxide (CeO2) nanoparticles and human serum albumin (HSA) form conjugates. These interactions do not impair HSA
Area of Science:
- Nanomaterials Science
- Biochemistry
- Biophysics
Background:
- Inorganic nanomaterials interact with biological fluids, forming protein coronas that alter nanoparticle and biomolecule properties.
- Cerium dioxide (CeO2) nanoparticles are versatile nanozymes with potential biomedical applications.
- Understanding nanoparticle-biomolecule interactions is crucial for nanomedicine development.
Purpose of the Study:
- To investigate the interaction between nanoscale cerium dioxide (CeO2) and human serum albumin (HSA).
- To assess the impact of CeO2 nanoparticles on HSA structure, function, and drug-binding capabilities.
Main Methods:
- Fourier transform infrared spectroscopy (FTIR)
- MALDI-TOF mass spectrometry
- UV-vis spectroscopy
- Fluorescence spectroscopy
Main Results:
- Confirmed the formation of human serum albumin-cerium dioxide (HSA-CeO2) nanoparticle conjugates.
- Observed changes in protein conformation dependent on CeO2 nanoparticle concentration (citrate-stabilized and pristine).
- Found no impairment of albumin drug-binding sites or HSA transport function.
Conclusions:
- CeO2 nanoparticle-HSA interactions form conjugates without compromising HSA's drug transport function.
- These findings are vital for understanding the biological fate of CeO2 nanoparticles.
- Informed nanobiomaterial engineering for enhanced efficacy and reduced side effects.
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