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Exchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy.

Alvaro Ruiz-Arias1, Francisco Fueyo-González2,3, Carolina Izquierdo-García2

  • 1Nanoscopy-UGR Laboratory. Departamento de Fisicoquímica, Unidad de Excelencia de Química Aplicada a Biomedicina y Medioambiente, Facultad de Farmacia, Universidad de Granada, Campus Cartuja, 18071, Granada, Spain.

Angewandte Chemie (International Ed. in English)
|November 22, 2023
PubMed
Summary

Researchers developed a new lanthanide antenna (PAnt) to reduce photobleaching in photoluminescence lifetime imaging microscopy (PLIM). This innovation enhances quantitative biological imaging by ensuring a continuous supply of intact dyes during long acquisitions.

Keywords:
BioimagingLanthanide AntennaLuminescencePLIMPhotostability

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Area of Science:

  • Bioimaging
  • Photoluminescence Spectroscopy
  • Lanthanide Chemistry

Background:

  • Lanthanides exhibit unique photoluminescence (PL) properties, including long lifetimes, making them suitable for biological imaging.
  • Photoluminescence lifetime imaging microscopy (PLIM) offers quantitative advantages but is hampered by photobleaching of lanthanide emissions during prolonged imaging.
  • Existing lanthanide complexes for bioimaging suffer from photobleaching, limiting their utility in live-cell applications.

Purpose of the Study:

  • To design and synthesize a novel, water-soluble lanthanide antenna (PAnt) to mitigate photobleaching in PLIM.
  • To investigate the efficacy of PAnt in self-assembled lanthanide complexes for reducing photobleaching during cellulo imaging.
  • To establish a new strategy for enhancing quantitative biological imaging using exchangeable lanthanide dyes.

Main Methods:

  • Synthesis of 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-phosphonic acid (PAnt) as an exchangeable lanthanide antenna.
  • Formation of self-assembled lanthanide complexes using PAnt for dynamic interaction with lanthanide ions.
  • Evaluation of photobleaching reduction in PLIM microscopy in cellulo compared to established lanthanide cryptates.

Main Results:

  • The developed PAnt is a small, aqueous-soluble molecule designed for dynamic interaction with lanthanide ions.
  • Self-assembled lanthanide complexes with PAnt demonstrated a significant reduction in photobleaching during PLIM acquisition.
  • The PAnt-based system showed superior photobleaching resistance compared to conventional lanthanide cryptates used in bioimaging.

Conclusions:

  • Exchangeable lanthanide antennas offer a viable strategy to overcome photobleaching limitations in PLIM.
  • The PAnt system provides a continuous replenishment of intact dye, enabling prolonged and quantitative imaging.
  • This approach opens new avenues for advanced quantitative photoluminescence lifetime imaging microscopy in biological research.