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Updated: Jul 9, 2025
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Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
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[Advances in the application of yeast surface display technology]
Lili Zhao1, Bingkai Su1, Shushu DU1
1Department of Immunology, School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang 471023, Henan, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|November 28, 2023
Summary
Yeast surface display (YSD) enables engineering of proteins like antibodies and enzymes by displaying them on yeast cells. This technology advances protein engineering for biotechnology and biomedicine.
Area of Science:
- Biotechnology
- Protein Engineering
- Molecular Biology
Background:
- Yeast surface display (YSD) is a powerful technology for protein engineering.
- It involves fusing target protein genes with yeast vectors for surface expression.
- The α-agglutinin system is a common YSD method.
Approach:
- YSD leverages yeast's eukaryotic post-translational modification for correct protein folding.
- This allows display of diverse proteins, including antibodies, receptors, enzymes, and peptides.
- The technology facilitates improvements in protein affinity, specificity, function, and stability.
Key Points:
- Recent advances in YSD applications for library construction and screening.
- Applications in antibody engineering, including affinity and specificity enhancement.
- Progress in enzyme engineering, protein engineering, and vaccine development using YSD.
Conclusions:
- YSD is a versatile tool with significant impact in biotechnology and biomedicine.
- It enables the optimization of various protein properties for diverse applications.
- Continued advancements in YSD promise further innovation in protein-based technologies.
