Engineering Crystallinity Gradients for Tailored CaO₂ Nanostructures: Enabling Alkalinity-Reinforced Anticancer...

CaO₂ nanoparticles (CNPs) can produce toxic Ca²⁺ and H₂O₂ under acidic pH, which accounts for their intrinsic anticancer activity but at the same time raises safety concerns upon systemic exposure. Simultaneously realizing minimized Ca²⁺/H₂O₂ production and enhanced anticancer activity poses a dilemma. Herein, we introduce a "crystallinity gradient-based selective etching" (CGSE) strategy, which is realized by creating a crystallinity gradient in a CNP formed by self-assembled nanocrystals. The nanocrystals distributed in the outer layer have a higher crystallinity and thus are chemically more robust than those distributed in the inner layer, which can be selectively etched. CGSE not only leads to CNPs with tailored single- and double-shell hollow structures and metal-doped compositions but more surprisingly enables significantly enhanced anticancer activity as well as tumor growth inhibition under limited Ca²⁺/H₂O₂ production, which is attributed to an alkalinity-reinforced lysosome-dependent cell death pathway.