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Association of Acute Hyperglycemia and Diabetes Mellitus with Platelet-derived Microparticle (PDMP) Levels During
Hana Anindya Indana1, Ira Puspitawati2, Dyah Samti Mayasari3,4
1School of Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada - Dr. Sardjito Hospital, Yogyakarta, Indonesia.
Objectives:
This research investigates whether there is an association between acute hyperglycemia and diabetes mellitus and the level of circulating platelet-derived microparticles (PDMPs) during an initial episode of acute myocardial infarction (AMI).
Methodology:
This was a cross-sectional study involving hospitalized AMI patients. Demographic and clinical data were obtained from hospital records. Diabetes mellitus was defined by the history of the disease, anti-diabetes medication use and/ or level of HbA1C ≥6.5%. Levels of HbA1c, admission random and fasting blood glucose levels were measured. Flow-cytometry method was used to determine the levels of PDMPs from collected venous blood through tagging with CD-41 FITC and CD-62 PE markers and a threshold size of <1 μm. The number of circulating PDMPs was compared according to glucometabolic state, namely acute hyperglycemia (admission random glucose ≥200 mg/dL and fasting glucose ≥140 mg/dL) and diabetes mellitus. The comparative analysis between groups was conducted with Student T-test or Mann-Whitney test, where applicable.
Results:
A total of 108 subjects were included and their data analyzed. The level of circulating PDMPs was significantly lower in subjects with admission random glucose ≥200 mg/dL as compared to those with below level [median (interquartile range (IQR)]: 2,710.0 (718.0-8,167.0) count/mL vs. 4,452.0 (2,128.5-14,499.8) count/mL, p = 0.05) and in subjects with fasting glucose ≥140 mg/dL as compared to those with below level (median (IQR): 2,382.0 (779.0-6,619.0) count/mL vs. 5,972.0 (2,345.7-14,781.3) count/mL, p = 0.006). The level of circulating PDMPs was also significantly lower in patients with diabetes mellitus as compared to those without (median (IQR): 2,655.0 (840.0-5,821.0) count/mL vs. 4,562.0 (2,128.5-15,055.8) count/mL; p = 0.007).
Conclusion:
Acute hyperglycemia and diabetes mellitus are significantly associated with a lower circulating PDMP level during an initial AMI episode.
Insights
Acute hyperglycemia and diabetes mellitus are linked to lower levels of circulating platelet-derived microparticles (PDMPs) in patients experiencing their first acute myocardial infarction (AMI). This finding suggests a potential role for PDMPs in the context of diabetes and heart attack.
Area of Science:
- Cardiology
- Endocrinology
- Hematology
Background:
- Platelet-derived microparticles (PDMPs) are cell fragments released from activated platelets.
- Elevated blood glucose levels and diabetes mellitus are known risk factors for cardiovascular diseases, including acute myocardial infarction (AMI).
- The role of PDMPs in the context of acute hyperglycemia and diabetes during AMI is not fully understood.
Purpose of the Study:
- To investigate the association between acute hyperglycemia and diabetes mellitus.
- To determine the levels of circulating platelet-derived microparticles (PDMPs) in patients during an initial episode of acute myocardial infarction (AMI).
Main Methods:
- Cross-sectional study of 108 hospitalized AMI patients.
- Diabetes mellitus defined by history, medication, or HbA1c ≥6.5%.
- PDMP levels measured via flow cytometry; acute hyperglycemia defined by blood glucose thresholds.
Main Results:
- Circulating PDMP levels were significantly lower in patients with acute hyperglycemia (random glucose ≥200 mg/dL or fasting glucose ≥140 mg/dL).
- Patients with diabetes mellitus exhibited significantly lower PDMP levels compared to those without diabetes.
- Specific median (IQR) PDMP counts were provided for comparison between groups.
Conclusions:
- Acute hyperglycemia is associated with reduced circulating PDMP levels during an initial AMI.
- Diabetes mellitus is also significantly associated with lower circulating PDMP levels in the context of AMI.
- These findings highlight a potential link between glucose dysregulation and PDMP levels in AMI patients.
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