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Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
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Two new phenolic amides from Allium chinense
Xiaoqing Li1, Shikai Yan1, Jihong Lu1
1Department of Natural Product Chemistry, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Chinese Herbal Medicines
|December 14, 2023
Summary
Two new phenolic amides were isolated from Allium chinense bulbs. Compound 3-indolcarbaldehyde demonstrated significant protection for myocardial cells against oxidative damage in vitro.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Cardiovascular Research
Background:
- Allium chinense, commonly known as Chinese onion, is traditionally used for medicinal purposes.
- Phenolic amides are a class of compounds with diverse biological activities.
- Myocardial injury is a significant health concern, necessitating the search for novel protective agents.
Purpose of the Study:
- To isolate and identify phenolic amides from Allium chinense bulbs.
- To evaluate the potential of these compounds in protecting myocardial cells from damage.
Main Methods:
- Phytochemical analysis involving silica gel and Sephadex LH-20 column chromatography, and High-Performance Liquid Chromatography (HPLC).
- Structural elucidation using Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry.
- In vitro assessment of myocardial protective effects using H9c2 cells exposed to hydrogen peroxide (H2O2).
Main Results:
- Two novel phenolic amides, alichinemide I (1) and alichinemide II (2), were isolated, along with six known compounds.
- The known compounds were identified as 3-indolcarbaldehyde (3), 1-(2-aminophenyl)urea (4), 2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid (5), N-trans-feruloyltyramine (6), N-trans-p-coumaroyltyramine (7), and N-(3,4-dimethoxyphenethyl)acetamide (8).
- Compound 3 (3-indolcarbaldehyde) at 50 μmol/L significantly inhibited H9c2 cell damage induced by H2O2.
Conclusions:
- Allium chinense bulbs contain novel and known phenolic amides.
- 3-Indolcarbaldehyde (3) exhibits significant in vitro myocardial protective activity.
- This study identifies 3-indolcarbaldehyde as a potential therapeutic constituent from Allium chinense for myocardial protection.
Keywords:
3-indolcarbaldehydeAllium chinensealichinemide Ialichinemide IImyocardium protective activitiesphenolic amidesMore Related Videos
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