Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

851
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
851
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

745
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
745
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

751
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
751
Mass Spectrometers01:16

Mass Spectrometers

5.5K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.5K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

626
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
626
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.2K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spontaneous Room-Temperature Polymerization of Carbon Disulfide in Water Microdroplets.

Journal of the American Chemical Society·2026
Same author

Transcending Structural Dependencies: A Tunable Mass Spectrometry-Driven Machine Learning Framework for Genotoxicity Prediction.

Environmental science & technology·2026
Same author

Noncontact Extraction Mass Spectrometry with Bubble Extraction Ionization Facilitates Operando Monitoring Electrochemical Reactions.

Analytical chemistry·2026
Same author

Direct Current Arc Plasma-Excited Nebulizer Gas-Assisted Electrospray Ionization Mass Spectrometry.

Analytical chemistry·2026
Same author

A Highly Sensitive and High-Throughput Quantitative HILIC-MS/MS Method for Systematic Profiling of RNA Modifications.

Analytical chemistry·2026
Same author

Oxidation of Organic Sulfur at the Air-Water Interface of Microdroplets: A New Way of Atmospheric Particle Precursor Formation.

ACS environmental Au·2026

Related Experiment Video

Updated: Jul 6, 2025

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.5K

Arc-Induced Electrospray Ionization Mass Spectrometry.

Kaineng Huang1, Hui Zeng1, Xingyue Li1

  • 1College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, Sichuan 610068, P. R. China.

Analytical Chemistry
|December 28, 2023
PubMed
Summary

Arc-induced electrospray ionization mass spectrometry (AESI-MS) offers a novel soft ionization technique. This method simplifies mass spectra and enhances ionization efficiency for diverse analytes, including amino acids.

More Related Videos

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.4K

Related Experiment Videos

Last Updated: Jul 6, 2025

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.5K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.4K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Plasma Physics

Background:

  • Traditional electrospray ionization (ESI) faces limitations with complex matrices and certain analyte types.
  • Atmospheric pressure chemical ionization (APCI) and ESI are established soft ionization methods.
  • Developing novel ionization techniques is crucial for expanding mass spectrometry applications.

Purpose of the Study:

  • To introduce and characterize Arc-induced Electrospray Ionization Mass Spectrometry (AESI-MS) as a new soft ionization technique.
  • To demonstrate the advantages of AESI-MS over conventional ESI for various analytical challenges.
  • To explore the potential of AESI-MS for analyzing diverse compounds, including amino acids in complex samples.

Main Methods:

  • Development of an AESI source utilizing alternating current arc plasma for aerosol generation.
  • Integration of the AESI source with a mass analyzer for analyte ionization and detection.
  • Comparative analysis of AESI-MS with ESI-MS for ionization efficiency, spectral simplicity, and salt tolerance.
  • Application of AESI-MS for the quantitative analysis of amino acids in complex biological matrices.

Main Results:

  • AESI-MS effectively generates charged microdroplets through arc plasma, enabling soft ionization of analytes.
  • The technique combines mechanisms of APCI and ESI, facilitating ionization of compounds with wide polarities.
  • AESI-MS demonstrates simplified mass spectra, high salt tolerance, and superior ionization efficiency compared to ESI.
  • Reliable quantitative analysis of amino acids in complex matrices was achieved using AESI-MS.

Conclusions:

  • AESI-MS is a versatile and efficient soft ionization technique with significant advantages over traditional ESI.
  • The method broadens microdroplet generation methodologies and offers a robust framework for novel electrospray ionization techniques.
  • AESI-MS shows great promise for direct analysis of amino acids and other challenging analytes in complex samples.