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High-throughput mass spectrometry analysis using immediate drop-on-demand technology coupled with an open port
Vilmos Kertesz1, Dana L Carper1, John F Cahill1
1Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Rapid Communications in Mass Spectrometry : RCM
|January 11, 2024
Summary
A new software enhances immediate drop-on-demand (I.DOT) technology with open port sampling interface (OPSI) for high-throughput mass spectrometry (MS). This automated system achieves rapid sample analysis, improving drug discovery and reaction monitoring.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Automation Technology
Background:
- The throughput of immediate drop-on-demand (I.DOT) technology with open port sampling interface (OPSI) is limited by software communication.
- High-throughput mass spectrometry (MS) analysis is crucial for applications like drug discovery.
Purpose of the Study:
- To develop novel software for flexible sample selection and maximized analysis throughput using I.DOT/OPSI-MS coupling.
- To overcome the limitations of current I.DOT/OPSI systems for enhanced MS analysis.
Main Methods:
- Developed a novel software for flexible sample selection from 96-well plates.
- Utilized I.DOT/OPSI coupled with MS for high-throughput analysis.
- Demonstrated chemical reaction monitoring using 2,3-diaminonaphthalene (DAN) and nitrite.
Main Results:
- Achieved a maximum throughput of 1.54 seconds/sample (7.41 min/96-well plate).
- Observed a relative standard deviation of 10 ± 1% across tested sampling strategies.
- Successfully monitored the chemical reaction of DAN to 2,3-naphthotriazole (NAT) with 2 seconds/sample throughput.
Conclusions:
- Developed a noncontact, automated I.DOT/OPSI-MS system with significantly improved throughput.
- The system's speed and precision make it suitable for drug discovery and in situ reaction monitoring.
- Optimized software interface is key to achieving enhanced analysis capabilities.
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