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Related Experiment Video

Updated: Jul 5, 2025

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Published on: August 16, 2013

Live-Cell Fluorescence Imaging for Virus-Host Interactions.

Francesca J Scribano¹, Kristen A Engevik¹, J Thomas Gebert¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 24, 2024

Summary

This summary is machine-generated.

Advancements in microscopy enable long-term live-cell fluorescence imaging of virus-infected cells. This technique tracks host-pathogen interactions and cellular responses with high temporal resolution.

Keywords:

Cell Fluorescence Host-pathogen Live-cell imaging Microscopy Virus

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Last Updated: Jul 5, 2025

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Published on: October 29, 2020

Area of Science:

Microscopy and Imaging Technologies
Cell Biology
Virology

Background:

Technological progress in microscopy enhances the study of host-pathogen interactions.
Engineered biosensors and improved microscope hardware address challenges in live-cell imaging.
Fluorescence microscopy offers high temporal resolution for observing cellular signaling and protein localization.

Purpose of the Study:

To present a detailed protocol for long-term live-cell fluorescence imaging.
To enable detailed observation of virus-infected cell lines over extended periods.
To facilitate the assessment of cellular responses to infection and potential therapeutic agents.

Main Methods:

Utilizing advanced fluorescence microscopy techniques.
Implementing engineered biosensors for enhanced signal detection.
Developing a protocol for sustained live-cell imaging of infected cell cultures.

Main Results:

Successful long-term live-cell imaging of virus-infected cell lines was achieved.
The protocol allows for the detection of dynamic cellular events.
High temporal resolution imaging provides insights into host-pathogen interplay.

Conclusions:

The described protocol is effective for long-term live-cell fluorescence imaging of virus-infected cells.
This method advances the study of host-pathogen interactions and cellular dynamics.
Enables precise assessment of infection effects and therapeutic interventions.