Quantitative mass spectrometry imaging: therapeutics & biomolecules.
Joseph H Holbrook1,2, Gabrielle E Kemper2, Amanda B Hummon1,2,3
1Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA. hummon.1@osu.edu.
Chemical Communications (Cambridge, England)
|January 29, 2024
Summary
Quantitative mass spectrometry imaging (qMSI) is advancing rapidly, enabling precise spatial mapping of biomolecules like lipids and peptides. This review covers recent qMSI progress and experimental considerations for biological sample analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Imaging
Background:
- Mass spectrometry imaging (MSI) is a powerful technique for label-free spatial analysis of thousands of molecules.
- Traditionally viewed as qualitative, MSI is evolving towards quantitative applications (qMSI).
- Advancements in instrumentation, sample preparation, and standards are driving the shift to qMSI.
Purpose of the Study:
- To review recent progress in quantitative mass spectrometry imaging (qMSI).
- To focus on qMSI applications for therapeutics, lipids, and peptides/proteins.
- To provide experimental considerations for conducting qMSI studies on biological samples.
Main Methods:
- Review of recent literature on quantitative mass spectrometry imaging.
- Analysis of advancements in instrumentation and sample preparation for qMSI.
- Discussion of standards development for qMSI.
Main Results:
- Recent advancements have made quantitative MSI (qMSI) more feasible and common.
- The review highlights progress in qMSI for analyzing therapeutics, lipids, and peptides/proteins.
- Detailed experimental considerations for biological qMSI studies are presented.
Conclusions:
- Quantitative MSI is a rapidly developing field with significant potential.
- The review provides a roadmap for researchers aiming to advance qMSI methodologies.
- This work aims to facilitate more robust and quantitative molecular imaging studies.
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