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Impact of the Different Chemical-Based Decellularization Protocols on the Properties of the Caprine Pericardium
Thirumalai Deepak1, Deepak Bajhaiya1, Anju R Babu2
1Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India.
Purpose:
This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations.
Methods:
Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100.
Results:
Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus (p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue.
Conclusion:
The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations.
