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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Exercise-Induced MYC as an Epigenetic Reprogramming Factor That Combats Skeletal Muscle Aging
Ronald G Jones1, Ferdinand von Walden2, Kevin A Murach1
1Exercise Science Research Center, Molecular Muscle Mass Regulation Laboratory, Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, AR.
Exercise and Sport Sciences Reviews
|February 23, 2024
Summary
Exercise activates the c-Myc (Myc) gene in skeletal muscle. This study hypothesizes that exercise-induced MYC protein pulses can epigenetically reprogram muscle for improved resilience and function.
Area of Science:
- Molecular biology
- Exercise physiology
- Epigenetics
Background:
- The Yamanaka factors (Oct3/4, Sox2, Klf4, and c-Myc) are key for cellular reprogramming.
- Skeletal muscle adaptation to exercise involves complex molecular signaling pathways.
- Understanding exercise-induced molecular changes is crucial for enhancing muscle function.
Purpose of the Study:
- To investigate the role of the transcription factor c-Myc (Myc) in skeletal muscle adaptation to exercise.
- To test the hypothesis that pulsatile MYC induction after exercise can epigenetically reprogram skeletal muscle.
Main Methods:
- Analysis of "Yamanaka factors" expression in skeletal muscle following exercise.
- Focus on the responsiveness and localization of c-Myc (Myc) within muscle fibers.
- Investigating the potential of exercise-induced MYC protein pulses for epigenetic reprogramming.
Main Results:
- The transcription factor c-Myc (Myc) demonstrates the highest responsiveness to exercise in skeletal muscle.
- c-Myc (Myc) protein is found to be enriched within muscle fibers after exercise.
- Pulsatile MYC induction is proposed as a mechanism for exercise-mediated epigenetic changes.
Conclusions:
- Exercise significantly impacts c-Myc (Myc) levels in skeletal muscle.
- The study posits a novel mechanism where exercise-induced MYC protein influences skeletal muscle epigenetics.
- This reprogramming may lead to enhanced muscle resilience and improved functionality.
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