Towards developing a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) compatible
Li-Cyun Chen1, Chuping Lee2, Cheng-Chih Hsu1
1Department of Chemistry, National Taiwan University, No.1, Sec. 4, Roosevelt Road, Taipei City, 106319, Taiwan.
Analytica Chimica Acta
|March 4, 2024
Summary
Researchers developed a new method combining expansion microscopy with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). This technique enhances spatial resolution for detailed molecular mapping in tissues.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microscopy
Background:
- Mass spectrometry imaging (MSI) offers molecular distribution insights but lacks spatial resolution for detailed morphology.
- Expansion microscopy improves optical imaging resolution but faces challenges when adapted for MSI.
Purpose of the Study:
- To develop a novel protocol integrating expansion microscopy with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI).
- To overcome limitations of combining expansion microscopy and MSI, such as sample fragility and molecule loss.
Main Methods:
- A modified sample preparation protocol was developed for tissue expansion compatible with MALDI MSI.
- The expanded samples were analyzed using MALDI MSI for molecular distribution and spatial resolution assessment.
Main Results:
- Successfully combined expansion microscopy with MALDI MSI, enhancing spatial resolution.
- The developed protocol addresses challenges like sample fragility and vacuum stability for MSI analysis.
- Improved visualization of tissue morphology and molecular localization was achieved.
Conclusions:
- The developed MALDI MSI-compatible expansion protocol significantly improves MSI spatial resolution.
- This technique holds potential for detailed molecular and morphological analysis in various biological tissues.
- Facilitates better understanding of tissue architecture and molecular landscapes in diseases.
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