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One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

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Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array.

Han Zhang¹, Junsong Mou¹, Jiawang Ding²

¹CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Analytica Chimica Acta

|March 4, 2024

Summary

This summary is machine-generated.

This study introduces a novel antibiotic screening method using bacterial apoptosis, specifically phosphatidylserine (PS) exposure, to detect bacterial death. This approach offers a sensitive, rapid, and general method for antibiotic susceptibility testing.

Keywords:

Antibiotic screening Bacteria apoptosis Peptide Potentiometric detection

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Area of Science:

Biotechnology
Microbiology
Analytical Chemistry

Background:

Phenotypic antimicrobial susceptibility testing is crucial but requires diverse strategies for different bactericidal mechanisms.
Bacterial apoptosis, a distinct cell death pathway, has not been utilized for antibiotic screening.
Developing new methods for rapid and reliable antibiotic screening is essential for combating antimicrobial resistance.

Purpose of the Study:

To develop a novel antibiotic screening method based on detecting antibiotic-induced phosphatidylserine (PS) exposure in apoptotic bacteria.
To explore the utility of E. coli PS externalization as a general apoptosis marker for various antibacterial agents.
To establish a sensitive, rapid, and generalizable platform for antibiotic susceptibility testing.

Main Methods:

Developed a method utilizing a positively charged peptide immobilized on magnetic beads (MBs) to capture apoptotic E. coli via PS exposure.
Employed a magneto-controlled polymeric membrane potentiometric sensor to detect charge changes upon capture of apoptotic bacteria.
Validated the system for screening different classes of antibiotics and silver nanoparticles against E. coli.

Main Results:

Successfully demonstrated the detection of antibiotic-induced PS exposure in E. coli as a marker of bacterial apoptosis.
Achieved sensitive and generalizable antibiotic screening within 1.5 hours using the developed potentiometric sensor array.
Showcased the method's effectiveness for various antibiotics and silver nanoparticles, indicating broad applicability.

Conclusions:

Antibiotic-induced PS exposure in bacteria can serve as a reliable indicator for antibiotic screening.
The developed magnetic bead-based potentiometric sensor system offers a sensitive, rapid, and generalizable approach for antibiotic susceptibility testing.
This method presents a promising new avenue for phenotypic antibiotic susceptibility testing, potentially aiding in the fight against antimicrobial resistance.