Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 1, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.6K

Microfluidic Impedance Cytometry Enabled One-Step Sample Preparation for Efficient Single-Cell Mass Spectrometry.

Junwen Zhu1, Siyuan Pan2, Huichao Chai1

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.

Small (Weinheim an Der Bergstrasse, Germany)
|March 14, 2024
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Label-Free Dual-Mode Flow Cytometry for Single-Cell Physicochemical Property Analysis.

Analytical chemistry·2026
Same author

DLD-based on-chip sample handling for label-free single-cell MS cytometry.

Analytical methods : advancing methods and applications·2025
Same author

Neuromorphic-enabled video-activated cell sorting.

Nature communications·2024
Same author

Liquid metal electrodes enabled cascaded on-chip dielectrophoretic separation of large-size-range particles.

Lab on a chip·2024
Same author

Capillarity Enabled Large-Array Liquid Metal Electrodes for Compact and High-Throughput Dielectrophoretic Microfluidics.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

Single-shot deterministic complex amplitude imaging with a single-layer metalens.

Science advances·2024

A novel microfluidic chip enables one-step sorting and desalting of single cells for mass spectrometry (MS). This impedance flow cytometry (IFC) method streamlines sample preparation, improving efficiency and enabling direct MS analysis.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Single-cell mass spectrometry (MS) requires efficient sample preparation, including cell sorting and desalting.
  • Traditional methods are often complex, require multiple apparatus, or have insufficient performance.

Purpose of the Study:

  • To develop a one-step sample preparation strategy for single-cell MS.
  • To integrate cell sorting and desalting using microfluidics.

Main Methods:

  • Utilized label-free impedance flow cytometry (IFC) based microfluidics for single-cell characterization and sorting.
  • Developed a microfluidic chip for simultaneous sorting and transferring cells to MS-compatible solutions.

Main Results:

Keywords:
impedance flow cytometrymass spectrometrysample preparationsingle‐cell analysissorting and desalting

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.1K

Related Experiment Videos

Last Updated: Jul 1, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.6K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.1K
  • Achieved high sorting efficiency (>99%) and desalting efficiency (>99%).
  • Demonstrated high cancer cell purity (≈87%) in sorted samples.
  • Verified the workflow for separating and analyzing normal (MCF-10A) and cancer (MDA-MB-468) cells via MS.

    • Conclusions:

    • The proposed IFC microfluidic chip offers an efficient, one-step solution for single-cell sample preparation for MS.
    • This technology streamlines the workflow, enabling direct MS analysis and paving the way for multi-modal single-cell characterization.