Related Experiment Video
Updated: Jun 30, 2025
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Published on: April 1, 2017
13.9K
Probing Protein Complexes Composition, Stoichiometry, and Interactions by Peptide-Based Mass Spectrometry
1MRC Laboratory of Molecular Biology, Cambridge, UK. gdeglies@mrc-lmb.cam.ac.uk.
Advances in Experimental Medicine and Biology
|March 20, 2024
Summary
Mass spectrometry characterizes protein complexes by identifying subunits and quantifying their ratios. Advanced techniques like cross-linking and hydrogen/deuterium exchange provide structural insights into these biological assemblies.
Area of Science:
- Biochemistry
- Proteomics
- Analytical Chemistry
Background:
- Protein complexes are crucial for cellular functions.
- Mass spectrometry is a powerful tool for analyzing protein complexes.
- Characterization can range from subunit identification to structural analysis.
Purpose of the Study:
- To detail the application of peptide-based LC-MS/MS for protein complex characterization.
- To cover qualitative, quantitative, and structural aspects of complex analysis.
- To highlight methods for subunit identification, stoichiometry, and structural investigation.
Main Methods:
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for qualitative and quantitative analysis.
- Cross-linking mass spectrometry for proximity information.
- Hydrogen/deuterium exchange for structural dynamics.
Main Results:
- Peptide-based LC-MS/MS enables subunit identification and stoichiometry determination.
- Quantitative analysis provides subunit ratios and copy numbers.
- Structural methods offer insights into the architecture of protein assemblies.
Conclusions:
- Peptide-based LC-MS/MS is versatile for comprehensive protein complex characterization.
- Combining qualitative, quantitative, and structural methods yields a deeper understanding.
- This approach is vital for investigating biological assemblies.
Keywords:
Cross-linking and mass spectrometryHydrogen/deuterium exchangeProtein identificationProteomicsQuantitative proteomicsStoichiometryMore Related Videos
Related Concept Videos
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
02:26Protein Networks
3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
01:33Proteomics
7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K