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Updated: Jul 8, 2026
11:16
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
16.2K
Green immobilization: Enhancing enzyme stability and reusability on eco-friendly support
Vesen Atiroğlu1, Atheer Atiroğlu1, Ahmed Atiroğlu2
1Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application and Research Center (BIMAS-RC), 54050, Sakarya, Turkey; Sakarya University, Biomaterials, Energy, Photocatalysis, EnzymeTechnology, Nan &Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R & D Group), 54050, Sakarya, Turkey.
Food Chemistry
|March 24, 2024
Summary
This study immobilizes catalase enzymes onto hyaluronic acid (HA) and chitosan-based materials. Immobilized catalase shows enhanced stability and reusability, offering sustainable solutions for various industrial applications.
Area of Science:
- Biochemistry and Materials Science
- Focus on green chemistry and sustainable materials
- Exploration of polysaccharides like hyaluronic acid (HA), chitin, and chitosan
Background:
- Growing demand for sustainable materials and green chemistry principles
- Need for robust enzyme immobilization techniques
- Potential of polysaccharides as enzyme supports
Purpose of the Study:
- To immobilize catalase enzymes onto various polysaccharide matrices
- To evaluate the structural and functional enhancements of immobilized catalase
- To explore the potential of these biocatalysts in industrial applications
Main Methods:
- Immobilization of catalase onto chitosan, chitin, HA/chitin, and HA/chitosan matrices
- Assessment of pH and temperature stability, thermal resilience, half-life, and storage durability
- Fourier-transform infrared (FTIR) spectroscopy for structural analysis
- Evaluation of enzyme reusability over multiple cycles
- Application of covalent cross-linking to enhance stability
Main Results:
- Catalase immobilized on HA/chitin and HA/chitosan matrices showed high efficacy (73.80% and 79.55%) after 25 cycles
- Covalent cross-linking significantly improved enzyme stability and recycling efficiency
- FTIR analysis confirmed successful enzyme immobilization and structural integrity
- Enhanced pH, temperature, and storage stability were observed
Conclusions:
- Hyaluronic acid and chitosan-based matrices are effective supports for catalase immobilization
- Immobilized catalase exhibits remarkable stability, durability, and reusability
- This innovation holds significant promise for sustainable industrial applications, including food, detergents, and bioremediation
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