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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
Published on: June 1, 2012
Fabrication of a 96-electrode array using carbon dioxide laser ablation
Supatinee Kongkaew1, Yudtapum Thipwimonmas2, Mareeyam Hayeeabu2
1Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
A novel 96 laser-induced multigraphene electrode microwell plate (96L-MGE-MP) was fabricated using laser ablation. This cost-effective, reproducible platform enables rapid, multichannel electrochemical detection for diverse analytes.
Area of Science:
- Electrochemistry
- Materials Science
- Microfluidics
Background:
- Microwell plates are essential for high-throughput screening.
- Developing integrated electrodes within microwells offers advantages for miniaturized electrochemical systems.
- Laser-induced graphene (L-GE) presents a versatile material for electrode fabrication.
Purpose of the Study:
- To describe the fabrication and characterization of a 96 laser-induced multigraphene electrode integrated microwell plate (96L-MGE-MP).
- To evaluate the electrochemical performance and reproducibility of the developed platform.
- To demonstrate its potential for multichannel electrochemical detection.
Main Methods:
- Fabrication of 96 electrochemical cells using laser ablation of polyimide adhesive tape to create laser-induced graphene electrodes (L-GE).
- Microwell creation via laser ablation of poly-methyl methacrylate (PMMA).
- Morphological and electrochemical characterization of L-GE, optimizing laser processing parameters (laser power-laser speed ratios).
Main Results:
- L-GE fabricated at optimal laser power-laser speed ratios (0.008-0.02 W s mm⁻¹) exhibited good electrochemical behavior and a surface roughness of 475.47 nm.
- The 96L-MGE-MP was fabricated in 24.2 minutes and demonstrated compatibility with various analytes.
- Electrocatalytic performance was investigated using 10 benchmark redox compounds via voltammetry.
- Multichannel detection was achieved by connecting the 96L-MGE-MP to a 96× connector, showing good reproducibility with relative standard deviation (RSD) below 5.3%.
Conclusions:
- The 96L-MGE-MP is a rapidly fabricated, reproducible, and versatile platform for electrochemical sensing.
- The developed method offers a cost-effective approach for creating integrated electrode-microwell systems.
- This technology holds promise for high-throughput, multichannel electrochemical analysis.
