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Published on: May 9, 2022
Injectable Thermosensitive Gel CH-BPNs-NBP for Effective Periodontitis Treatment through ROS-Scavenging and Jaw
Yuli Wang1, Yuqing Yuan2, Ruyu Wang1
1Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Centre of Stomatological Translational Medicine, Nanjing Medical University, Nanjing, Jiangsu, 210029, China.
This study developed an injectable gel to treat periodontitis by delivering black phosphorus nanosheets (BPNs) and dl-3-n-butylphthalide (NBP). The treatment reduced inflammation, protected cells from oxidative stress, and promoted bone regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Oral Biology
Background:
- Periodontitis involves inflammation and tissue damage driven by oxidative stress and reactive oxygen species (ROS).
- The jaw vascular unit (JVU), including blood and lymphatic vessels, is critical for fluid homeostasis and periodontal disease pathology.
- Current treatments for periodontitis often lack targeted delivery and comprehensive management of underlying pathological mechanisms.
Purpose of the Study:
- To develop an injectable, thermosensitive hydrogel (CH-BPNs-NBP) for localized delivery of ROS-scavenging black phosphorus nanosheets (BPNs) and lymphatic-promoting dl-3-n-butylphthalide (NBP).
- To evaluate the therapeutic efficacy of CH-BPNs-NBP in protecting against oxidative stress, modulating immune responses, and promoting tissue regeneration in periodontitis models.
Main Methods:
- Synthesis and characterization of an injectable thermosensitive hydrogel loaded with BPNs and NBP.
- In vitro assessment of the gel's ability to scavenge ROS and protect macrophages and lymphatic endothelial cells.
- In vivo evaluation of the therapeutic effects on inflammation, tissue damage, and alveolar bone regeneration in a periodontitis animal model.
Main Results:
- The CH-BPNs-NBP gel demonstrated controlled release of BPNs and NBP, effectively reducing oxidative stress.
- In vitro studies showed protection of cells from ROS, promotion of M2 macrophage polarization, and enhanced lymphatic function.
- In vivo studies revealed significant reduction in periodontal inflammation and tissue destruction, alongside substantial promotion of alveolar bone regeneration.
Conclusions:
- The injectable thermosensitive CH-BPNs-NBP gel represents a novel and effective therapeutic strategy for periodontitis.
- This approach successfully combines ROS scavenging and lymphatic function enhancement for comprehensive periodontal disease management.
- The findings highlight the potential of this biomaterial-based therapy for promoting oral tissue regeneration and treating inflammatory conditions.
